I'm sure there must be people who have encountered this problem (having to receive results at different times points and apply several compensation matrices to one flwoSet). wspt Workspace gating template from FlowJo (10.0.8) to open it & use it as my gating template in openCyto? (possibly with openWorkspace function or nay other way)? And, how do I compensate my flowSet, if every single sample/.fcs file of my flowSet has to have different compensation matrix? So, my questions would be: how can I use. fcs file (1 patient) in my Flowset has different compensation matrix (because we had the results of the patients on different weeks), I cannot write single spillover values in the pre-processing column of a gating template that i'm creating.
HOW TO APPLY NEW EDITED MATRIX TO SAMPLES IN FLOWJO 10 HOW TO
However, I don't know how to do the compensation (specify the compensation values) that are usually automatically given in the fcs metadata.Since almost every single. xml file, it gives the following error:Įrror in xpathApply(x,"/Workspace",function(x) xmlGetAttrx(x,"version")) ]: subscript out of bounds.Ģ) I also tried to create/use my own template as. xml file, but openWorkspace() function still does not open the file in any format.
![how to apply new edited matrix to samples in flowjo 10 how to apply new edited matrix to samples in flowjo 10](https://expert.cheekyscientist.com/wp-content/uploads/2019/06/adding-keywords-during-analysis-of-FlowJo-data-during-a-flow-cytometry-experiment-1.jpeg)
xls files, and I even converted my gating template to. My flowJo version only saves the workspace as. xml format file, which seems to be the only format that openWorkspace() function takes while opening the gating template
![how to apply new edited matrix to samples in flowjo 10 how to apply new edited matrix to samples in flowjo 10](https://expert.cheekyscientist.com/wp-content/uploads/2019/06/issues-with-the-Universal-Negative-in-FlowJo-software-flow-cytometry-analysis.jpeg)
![how to apply new edited matrix to samples in flowjo 10 how to apply new edited matrix to samples in flowjo 10](https://user-images.githubusercontent.com/25808102/109896537-ed414f00-7c45-11eb-8d9e-15b3ec4cefdb.png)
The problems are:ġ) FlowJo cannot save the workspace as. I am experiencing an issue while opening a FlowJo (version 10.0.8) Workspace file for using it as a gating template. I'm working with the openCyto package to compare manually (flowJo) analyzed clinical data with openCyto analysis. I am new to R and Bioconductor, but want to say thank you to the creators for sharing all the materials for free.